Seryl Transfer Ribonucleic Acid Synthetase from Bakers' Yeast
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چکیده
منابع مشابه
Purification and properties of lysyl transfer ribonucleic acid synthetase from bakers' yeast.
Lysyl transfer ribonucleic acid synthetase (L-lysine: tRNA ligase (AMP), EC 6.1.1.6) was pursed to a state of apparent homogeneity from bakers’ yeast. For each of two different preparation procedures, a 250to 500-fold purikation of the enzyme was obtained, and the final purification step yielded two distinct protein components with specific activity values of approximately 0.5 and 1.0 pmole of ...
متن کاملProtection of bovine seryl-transfer ribonucleic acid (seryl-tRNA) synthetase from chemical modification by its substrates, and some kinetic parameters.
phenylmethanesulfonyl fluoride, and appeared to be unnecessary for the recognition. However, the modification of Arg residues with phenylglyoxal, His residues with diethylpyrocarbonate and sulfhydryl groups with 5,5'-dithiobis(2-nitrobenzoic acid), N-ethylmaleimide, iodoacetic acid or iodoacetamide showed that these residues were essential for the tRNA recognition by SerRS. Protection experimen...
متن کاملCharacterization of two species of methionine transfer ribonucleic acid from bakers' yeast.
The biochemical properties of two species of methionine transfer ribonucleic acid from bakers’ yeast, separated by diethylaminoethyl Sephadex column chromatography, have been studied. Each species of the two methionine tRNAs, designated methionine tRNA I and methionine tRNA II, respectively, has been purified by benzoylated DEAEcellulose column chromatography. Methionine tRNA I can be esterifie...
متن کاملPurification and properties of the L-cysteinyl ribonucleic acid synthetase of bakers' yeast.
In this report a method is described for the puritication of the L-cystemyl-RNA synthetase of Bakers’ yeast. The enzyme, which was purified approximately 710-fold, was shown to have a molecular weight of approximately 160,000, and did not contain activity for any of the ammo acids commonly occurring in protein except L-cysteine. Optimum conditions for enzyme activity, such as substrate concentr...
متن کاملCytoplasmic Methionyl - tRNA Synthetase from Bakers ’ Yeast
Methionyl-tRNA synthetase has been purified from a yeast strain carrying the MESl structural gene on a high copy number plasmid (pFL1). The purified enzyme is a monomer of M, = 85,000 in contrast to its counterpart from Escherichia coli which is a dimer made up of identical subunits (M, = 76,000; Dardel, F., Fayat, G., and Blanquet, S . (1984) J. Bucteriol. 160,1115-1122). The yeast enzyme was ...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1968
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)93174-1